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Microvolume UV/ VIS (Nano) Spectrophotometer, SP-MUV1000, SP-MUV1000F

  • Microvolume UV/ VIS (Nano) Spectrophotometer, SP-MUV1000, SP-MUV1000F

Description

Micro Volume Spectrophotometer

It is an instrument used to detect DNA, RNA purity, concentration, and protein concentration. It can quickly measure the concentration of nucleic acid.We can use two detection modes: pedestal and cuvette, Quick, accurate and repeatable micro-measurement can be carried out without dilution.Please point the sample directly on the sample plate. No accessories such as cuvettes or capillaries are required. After the measurement, you can choose to wipe off the sample directly or use a pipette to recover the sample. The steps are simple and fast.

1. 7-inch high-definition capacitive touch screen, no need for computer connection, and it can be detected by a single machine.
2. Detection wavelength range: 190-850nm
3. Only 0.5—2μL sample is required for base measurement, and dilution is not required even for high concentration samples.
4. It has an OD600 optical path detection system and a cuvette mode, which is convenient for the detection of the concentration of bacteria, microorganisms and other culture solutions.
5. The closing of the detection arm can be detected automatically.
6. It has an extremely fast detection speed and can be detected at any time. The measurement of each sample is completed in a short period of time.
7. The sample does not need to be diluted, and the concentration range of the measurable sample is more than 100 times that of a conventional ultraviolet-visible photometer.
8. Easy-to-use data printing function, print reports directly through the built-in printer.
9. The service life of the xenon flash lamp can reach 1 billion times (up to 10 years). It can be used directly without preheating and can be tested at any time. No other consumables are required.
10. 100,000 pieces of data can be stored in each subdirectory.
11. The lowest detection limit in fluorometer mode can reach 0.5pg/μL (only for SP-MUV1000F).

Specifications

ModelSP-MUV1000SP-MUV1000F
Wavelength range190-850nm190-850nm
Sample capacity0. 5-2u I0. 5-2ul
Optical distance0. 05mmx 0. 2mm、 1.0mm  (automatic switchover)0. 05mmx 0. 2mm、 1.0mm
(automatic switchover )
Light source/lifeXenon lamp/109 timesXenon lamp/109 times
Detector2048 element linear CCD array2048 element linear CCD array
Spectral bandwidth2nm2nm
Absorbed light accuracy0. 003Abs0.003Abs
Absorbance accuracy±1% (7. 332Abs at 260nm)±1% (7. 332Abs at 260nm)
Absorbance range(Equivalent to 10mm) 0.04-300A(Equivalent to 10mm) 0.04-300A
Nucleic acid concentration range2ng/ μ L dsDNA~2ng/ μ L dsDNA~
15000ng/ μ L15000ng/ μ L
dsDNAdsDNA
Detection time≤5 s≤5 s
OD600Absorbance range0~4. 000 Abs0~4. 000 Abs
Absorbance stability[0,3 )≤0. 5%,[0,3 )≤0. 5%,
[3,4) ≤1%[3,4) ≤1%
Absorbance repeatability[0,3 )≤0. 5%,[0,3 )≤0. 5%,
[3,4) ≤1%[3,4) ≤1%
Absorbance accuracy[0,3)< 0.005A+1%; [3 ,4) ≤2%[0,3)< 0.005A+1%; [3 ,4) ≤2%
Fluorescence detection/Excitation wavelength: 460nm
Emission wavelength: 525nm
Fluorescence detectionLinearity/R2>0. 995
Repeatability/<1.5%
Stability/<1.5%
PrintBuilt-in thermal printerBuilt-in thermal printer
Data output modeUSBUSB
Material of sample baseQuartz optical fiber and high hard aluminumQuartz optical fiber and high hard aluminum
Input voltageVAC 100-240V ; VDC 24V 2AVAC 100-240V; VDC 24V 2A
Power<15W (Standby: 5W)<15W (Standby: 5W)
Dimensions(W*D*H)208*290*180mm208*290*180mm
Weight3.2 kg 3.2 kg

Reagents and applications corresponding to different fluorescence channels

ChannelExcitation WavelengthCommon Fluorescent ReagentsApplication
Blue channel(Standard configuration)460±20nmPicoGreen, oligreen, RiboGreenR, GFP, Protein, FluoresceindsDNA, ssDNA,

 

Green fluorescent protein GFP,

Gene detection, Fluorescein detection,

Protein quantification

Uv channel(optional)365±20nmHoechst 33258, 4-MU, EnZCheK CaspaseNucleic acid quantitation,

 

Plant GUS reporter gene detection,

Apoptosis detection

Green channel(optional)525±20nmRhodamine, Cy3, RFP Vybrant CytotoxicityRhodamine assay,

 

Cy-3 fluorescent label detection,

Red fluorescent protein RFP gene detection,

Cell viability toxicity assay

Red channel(optional)625±20nmCy5, Quant-iT RNACy-5 fluorescent label detection,

 

RNA quantification

 
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